BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt CAS:6578-06-9

BCIP-Toluidine, also known as 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt, is a chemical compound commonly used in molecular biology and biochemistry research. It is primarily used as a substrate in enzyme-linked immunosorbent assays (ELISAs) or immunohistochemical staining techniques to detect alkaline phosphatase activity.

When BCIP-Toluidine is cleaved by alkaline phosphatase, it undergoes a color change reaction, producing a blue-purple precipitate. This reaction allows for the visualization and quantification of alkaline phosphatase activity in samples. The intensity of the color can be measured spectrophotometrically and is directly proportional to the enzyme activity.

The use of BCIP-Toluidine as a substrate is especially common in applications involving DNA or RNA labeling, such as in situ hybridization (ISH) or Southern and Northern blotting. It provides a reliable and sensitive method for detecting the presence of target nucleic acids in samples.