Bis-tris hydrochloride CAS:124763-51-5

Buffering Agent: One of the main effects of bis-tris hydrochloride is its ability to maintain a stable pH. It acts as a buffer by resisting changes in pH when acids or bases are added to a solution. This effect makes it useful in many biochemical and biological experiments.

Protein Electrophoresis: Bis-tris hydrochloride is commonly used in protein electrophoresis techniques, such as SDS-PAGE. As part of the running buffer, it helps to create a suitable pH environment for the separation and analysis of proteins based on their molecular weight.

Enzyme Activity Assays: Bis-tris hydrochloride is often employed as a buffer in enzyme activity assays. It provides the optimal pH conditions for the enzyme to function properly, allowing accurate measurement of enzymatic activity and kinetics.

Cell Culture: In cell culture, bis-tris hydrochloride can be used as a buffering agent in media to maintain a stable pH for cell growth and viability. It helps to create an optimal environment for cells to grow, ensuring their proper functioning.

Pharmaceutical Formulations: Bis-tris hydrochloride is also used in some pharmaceutical formulations to adjust and maintain the pH of the product. It can be found in various liquid formulations, injectables, and topical preparations.