L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3

L-Leucine p-nitroanilide hydrochloride is commonly used as a substrate in biochemical research and enzyme assays to measure the activity of various enzymes, particularly proteases and peptidases. When these enzymes cleave the substrate, it releases p-nitroaniline, which can be detected spectrophotometrically.

The rate of the color change is directly proportional to the enzymatic activity, allowing for the quantification of enzyme activity in biological samples. This makes L-Leucine p-nitroanilide hydrochloride a useful tool for studying enzyme kinetics, screening for enzyme inhibitors or activators, and assessing the effects of experimental conditions or interventions on enzyme activity.

The sensitivity of L-Leucine p-nitroanilide hydrochloride in different enzymatic assays may vary, requiring optimization for specific systems. It is typically used at concentrations ranging from 0.5 mM to 5 mM, depending on the specific enzyme and experimental conditions.