Uridine,2′-deoxy-5-ethynyl- CAS:61135-33-9

5-ethynyl-2′-deoxyuridine (EdU) is a nucleoside analog that is commonly utilized in the field of molecular biology and cell biology research. It is frequently employed to label and detect newly synthesized DNA in live cells through a process known as “click chemistry.” This technique allows for visualization and quantification of DNA replication and cell proliferation. EdU has become a popular alternative to the traditional thymidine analog bromodeoxyuridine (BrdU) due to its compatibility with fluorescence microscopy and the absence of harsh DNA denaturation steps during detection. Additionally, EdU’s chemical structure allows for specific and efficient detection, making it a valuable tool for studying DNA synthesis and cell cycle dynamics.