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BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium salt CAS:102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium salt is a synthetic compound commonly used in biochemical research as a chromogenic substrate for detecting the activity of alkaline phosphatase enzymes. It is often referred to as BCIP for short.
When alkaline phosphatase acts upon BCIP, it cleaves off the phosphate group, resulting in the formation of a blue-purple precipitate. This color change allows researchers to visualize and quantify the presence of alkaline phosphatase in a sample.
BCIP is frequently used in various applications, including immunohistochemistry, protein and nucleic acid blotting techniques, and enzyme-linked immunosorbent assays (ELISAs).
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Iodoacetamide CAS:144-48-9 Manufacturer Price
Iodoacetamide is a chemical compound commonly used in biochemistry and molecular biology research. It is a thiol-reactive compound that specifically modifies cysteine residues in proteins by forming a covalent bond. This modification is often used in protein analysis techniques to block cysteine residues, such as in proteomics studies where it is utilized to selectively label and block cysteine residues prior to protein digestion and mass spectrometry analysis. Iodoacetamide is also used in protein structure studies and in the synthesis of labeled peptides.
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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt CAS:6578-06-9
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt is a chemical compound commonly used in molecular biology and biochemistry research. It is often used in enzyme-linked immunosorbent assays (ELISA) and protein labeling techniques.
This compound is a substrate for alkaline phosphatase, an enzyme widely used in molecular biology for detecting the presence of specific molecules or proteins through colorimetric or chemiluminescent reactions.
When the alkaline phosphatase enzyme is present, it cleaves the phosphate groups from 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, resulting in the formation of a blue or purple dye. This color change can be easily detected and quantified, enabling the measurement of enzyme activity or the presence of target molecules.
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N,N-Dimethyl-1,4-phenylenediamine dihydrochloride CAS:536-46-9
N,N-Dimethyl-1,4-phenylenediamine dihydrochloride is a chemical compound that is commonly referred to as DMPD dihydrochloride. It is a solid, crystalline substance that is soluble in water and other polar solvents.
DMPD dihydrochloride is often used in various chemical reactions and processes, including oxidation and reduction reactions. It can serve as a source of electrons or as an electron acceptor, making it useful in the synthesis of dyes, pharmaceuticals, and other organic compounds.
In addition to its reactivity, DMPD dihydrochloride is known for its strong reducing properties. It can be used as a reductant in analytical chemistry techniques, such as spectrophotometry, where it helps to quantify the concentration of certain substances.
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BSA CAS:9048-46-8 Manufacturer Price
Bovine albumin, also known as bovine serum albumin (BSA), is a protein derived from cows. It is commonly used in various applications in biology and biochemistry laboratories. Bovine albumin is often employed as a stabilizer and blocking agent in immunoassays, where it helps prevent non-specific binding of antibodies and other proteins. It can also serve as a nutrient source for cell cultures and as a carrier protein in molecular biology techniques, facilitating the binding and transport of small molecules. Bovine albumin is highly soluble in water and has a neutral pH, making it compatible with a wide range of experimental conditions.
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N,N’-Methylenebisacrylamide CAS:110-26-9
N,N’-Methylenebisacrylamide (MBAA) is a chemical compound commonly used in molecular biology and biochemistry applications. It is a crosslinking agent that is added to acrylamide gels to polymerize them and create a stable matrix for electrophoresis.
Crosslinking refers to the formation of chemical bonds between polymer chains, and MBAA plays a crucial role in this process. When added to acrylamide monomers, MBAA links them together to form a three-dimensional network of polymers. This crosslinked structure enhances the stability and strength of the gel, allowing for the separation of biomolecules based on their size, charge, or other physical properties.
MBAA is primarily utilized in techniques like polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), commonly used for protein and nucleic acid analysis. It helps in creating a uniform gel matrix through which the biomolecules can migrate during electrophoresis.
Aside from its use in gel electrophoresis, MBAA also finds applications in other areas such as molecular imprinting, chromatography, and drug delivery systems. It is known for its ability to create stable and mechanically robust polymer networks, making it a valuable tool in research and analytical laboratories.
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Butyrylthiocholine iodide CAS:1866-16-6
Butyrylthiocholine iodide is a compound commonly used in biological and biochemical research as a substrate in enzyme assays. It is often used to measure the activity of the enzyme butyrylcholinesterase (BChE). BChE is an enzyme found in various tissues and is involved in the hydrolysis of acetylcholine and other choline esters. By using butyrylthiocholine iodide as a substrate, researchers can determine the activity of BChE by measuring the release of thiocholine, which can be detected spectrophotometrically or fluorometrically.
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Ninhydrin hydrate CAS:485-47-2 Manufacturer Price
Ninhydrin hydrate is a chemical compound commonly used in forensic analysis and organic chemistry. It is a colorless or light yellow crystalline powder that is soluble in water and organic solvents.
Ninhydrin hydrate is known for its ability to react with amino acids and primary amines, producing a visible purple-blue color. This reaction is commonly used in forensic fingerprint analysis to visualize and enhance the visibility of latent fingerprints on porous surfaces. The ninhydrin solution is usually sprayed onto the surface, and the fingerprints develop over time as the ninhydrin reacts with the amino acids present in the fingerprint residues.
In organic chemistry, ninhydrin hydrate is used as a reagent for the detection and analysis of amino acids, peptides, and proteins. It can be used to determine the presence and quantify the amount of amino acids in a sample. The reaction with ninhydrin results in the formation of a colored product, which can be measured spectrophotometrically to estimate the concentration of the analyte.
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D-Luciferin CAS:2591-17-5 Manufacturer Price
D-Luciferin is a small molecule light-emitting compound primarily found in bioluminescent organisms such as fireflies, bacteria, and marine organisms. It is the key component of luciferase reaction, a biochemical process that generates light.
D-Luciferin is often used as a substrate in bioluminescence assays and imaging techniques, where its reaction with luciferase produces light that can be detected and quantified. This makes it a valuable tool in various research areas, including molecular biology, drug discovery, and biomedical imaging.
Due to its unique optical properties, D-Luciferin has been widely employed in studies involving gene expression, protein-protein interactions, and cellular signaling. It offers a non-invasive and sensitive method to investigate biological processes in real-time.
Additionally, D-Luciferin derivatives and analogs have been developed, allowing for various modifications and applications. These modified derivatives enable different wavelengths of light emission, enhanced stability, and improved tissue penetration.
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p-hydroxybenzoic acid,monosodium CAS:114-63-6
p-Hydroxybenzoic acid, monosodium (also known as sodium 4-hydroxybenzoate) is a chemical compound with the molecular formula C7H5NaO3. It is a sodium salt derived from p-hydroxybenzoic acid, which is a naturally occurring organic compound found in plants such as fruits and vegetables.
p-Hydroxybenzoic acid, monosodium is used in various industries for its antimicrobial properties. It is commonly employed as a preservative in food, beverages, cosmetics, and personal care products to inhibit the growth of bacteria, fungi, and yeasts. It helps prolong the shelf life of these products by preventing spoilage and preserving their quality.
In pharmaceutical applications, p-Hydroxybenzoic acid, monosodium is used as an excipient in oral medications and topical formulations. Its antimicrobial properties help maintain the stability and sterility of these products, ensuring their safety and efficacy.
Additionally, p-Hydroxybenzoic acid, monosodium has antioxidant properties, which make it useful in preventing oxidative damage in various products. It can help protect against the degradation of ingredients and extend the overall durability of the product.
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Dodecyl trimethyl ammonium bromide CAS:1119-94-4
Dodecyl trimethyl ammonium bromide, also known as DTAB or DTB, is a quaternary ammonium compound commonly used as a surfactant or detergent. It is a white, solid powder that is soluble in water.
DTAB is often utilized in various industries and applications, including personal care products, pharmaceuticals, and research laboratories. It can act as a cleaning agent, emulsifier, foaming agent, or wetting agent.
In personal care products, DTAB can be found in shampoos, conditioners, soaps, and body washes. It helps to create a lather, increase product stability, and improve the spreadability of the formulation.
In pharmaceuticals, DTAB is used in the formulation of various drug delivery systems, including microemulsions, nanoemulsions, and liposomes. It aids in drug solubilization and stabilization, enhancing drug absorption and effectiveness.
In research laboratories, DTAB is frequently employed in biological and biochemical studies. It can be used for membrane protein extraction and purification, DNA extraction, and gene delivery systems.
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Methylphenazinium methosulfate CAS:299-11-6
Methylphenazinium methosulfate (MPMS) is a redox-active compound that is commonly used as an electron carrier in various biochemical and biophysical studies. It is a salt consisting of a methylphenazinium cation (a heterocyclic compound) and a methosulfate anion.
MPMS is often used as an alternative to traditional electron carriers, such as ferricyanide or phenazine ethosulfate, due to its stability and high solubility in water. It possesses good redox properties, which allow it to accept and transfer electrons during enzymatic reactions.
One of the key applications of MPMS is in assays that involve the measurement of electron transfer or enzymatic activity. It is frequently used in conjunction with an enzyme system to monitor the transfer of electrons between different components. The reduction of MPMS can be detected spectrophotometrically, where its absorbance changes as a result of electron transfer processes.
MPMS is also utilized in studies related to mitochondrial respiration and oxidative phosphorylation. It can act as an artificial electron acceptor, allowing researchers to investigate the functioning and regulation of these processes in various biological systems.
